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Azote, levée et stress abiotique
(ACR)
- Mot(s) clé(s) :
Objet d'étude : arabidopsis, medicago truncatula
Question sociétale et finalité, contexte : amélioration génétique, changement climatique
Echelle d'étude : whole plant
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Objet d'étude : arabidopsis, medicago truncatula
Question sociétale et finalité, contexte : amélioration génétique, changement climatique
Echelle d'étude : whole plant
Localisation géographique : angers
Composé chimique, Facteur du milieu : acide aminé, alanine aminotransférase, glutamate déshydrogénase
Phénomène, processus et fonction : hypoxie, post levee, stress hydrique
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- Description détaillée :
Topics: (i) Regulation of primary root growth, a key step in post – germination growth and seedling Afficher la suite
Topics: (i) Regulation of primary root growth, a key step in post – germination growth and seedling establishment. (ii) Integrative functional genomics (transcriptomic and metabolomic) of young seedlings response to root – hypoxia and drought during the establishment phase.
Objectives
A/ We aim at pushing further the characterization of a double affinity nitrate transporter MtNRT1.1 which involvement in the control of primary root growth was revealed by quantitative genetic.
1. Exhaustive analysis of the properties of MtNRT1.1 as a transporter. (Dipeptide and dicarboxylate transport? Is the conserved phosphorylation site RXXT104 is involved in switching from low to high affinity transport properties? Expression in nodules and in other organs in the context of nodulated plants?)
2. Analysis of the properties of MtNRT1.1 as a nitrate sensor involved in nitrate signaling achieved by the characterizing TILLING mutants mtnrt1.1 (T385I and R552K) (specific interest in primary root growth and branching under nitrate-free and nitrate-containing media).
B/ We aim at acquiring further knowledge on the involvement of metabolic networks in the adaptive response of young seedlings to root – hypoxia (root water-logging) and drought.
1. Metabolic profiling and quantification of metabolome fluxes (fluxomic using 15N and 13C)
2. Approaches based on targeted genes and focused N and C metabolic pathways.

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